This is a Fluxion/Cell Microsystems BioFlux 1000z microfluidic system combined with a Zeiss LSM900 confocal microscope with Airyscan 2 for studying microbial biofilm growth. The equipment can be run as an integrated system, which enables monitoring spatial/temporal biofilm growth and the effect of external factors (e.g. antimicrobials) on biofilm structures in real time. This equipment can also be used separately; for example, growing microfluidic biofilms off the microscope, and using the microscope to study other samples.
The BioFlux instrument allows for growth of microbial biofilms on a range of substrate materials and under precise environmental control (e.g. temperature, shear flow, gas, mixed/gradient media input). It uses a well plate format, ranging from 6-well, 24-well to 48-well, with integrated microfluidics, for the delivery of different initial volumes of inlet media, the use of single or dual inlet channels.
The Zeiss LSM900 is equipped with 4 lasers (405/488/561/639nm) and 4 objectives (10x/20x/40xW/63xO/100xO), 2x GaAsP confocal detectors, 1x transmitted light detector as well as an Airyscan 2 detector. It also has fluorescence for visualisation of DAPI, GFP, RFP or associated fluorophores.
Facility staff
Reader in Microbial Structural Biology
Lecturer
| Equipment | UKRI | Charity | Industry |
Horizon 2020 EC |
Horizon Europe EC |
|
Zeiss LSM 900 (rate/hour) | 8.33 | 9.84 | 26.70 | 25.18 | 26.70 |
|
BioFlux 1000z (rate/day) | 48.56 | 54.12 | 87.96 | 84.95 | 87.96 |
| Consumables | Price (£/unit) |
| BioFlux Plates | 250.00 |
The BioFlux instrument allows for growth of microbial biofilms on a range of substrate materials and under precise environmental control (e.g. temperature, shear flow, gas, mixed/gradient media input). It uses a well plate format, ranging from 6-well, 24-well to 48-well, with integrated microfluidics, for the delivery of different initial volumes of inlet media, the use of single or dual inlet channels.
The Zeiss LSM900 is equipped with 4 lasers (405/488/561/639nm) and 4 objectives (10x/20x/40xW/63xO/100xO), 2x GaAsP confocal detectors, 1x transmitted light detector as well as an Airyscan 2 detector. It also has fluorescence for visualisation of DAPI, GFP, RFP or associated fluorophores.
Facility staff
Reader in Microbial Structural Biology
Lecturer
| Equipment | UKRI | Charity | Industry |
Horizon 2020 EC |
Horizon Europe EC |
|
Zeiss LSM 900 (rate/hour) | 8.33 | 9.84 | 26.70 | 25.18 | 26.70 |
|
BioFlux 1000z (rate/day) | 48.56 | 54.12 | 87.96 | 84.95 | 87.96 |
| Consumables | Price (£/unit) |
| BioFlux Plates | 250.00 |
Partners
Biotechnology and Biological Sciences Research Council
Contact us
For more information regarding this piece of equipment please contact the Academic Lead, James Garnett, using the details below