The Proteomics Facility is a Core Facility at King's College London, providing state-of-the-art protein mass spectrometry support for researchers at King's and external Institutions.
We are equipped with a comprehensive range of sophisticated instrumentation including an Orbitrap Fusion Lumos, protein search databases and protein/peptide data analysis software programs.
We endeavor to provide an excellent, prompt and value-for-money service. Our highly experienced staff, who have a vast knowledge of proteomics, can provide expert advice on planning experiments, understanding results obtained, grant writing and manuscript preparation.
Protein identification
Determination of protein content from simple or complex mixtures and immunoprecipitations.
Post-translational modifications (PTM)
Analysis of modifications such as phosphorylation and ubiquitination with site localisation.
Protein quantitation
Relative quantification of proteomes using label-free or stable isotope labelling (TMT).
Biomarker discovery
Global protein discovery from complex mixtures and targeted analysis.
Facility staff
Proteomics Facility Manager
Proteomics Deputy Facility Manager
Mass Spectrometry Technician, Proteomics
The Proteomics Facility is equipped with state-of-the-art mass spectrometry technology combined with experienced scientists that can deliver the highest quality proteomic results.
We can provide a range of services from the simplest protein identification to very complex large scale biomarker discovery and verification studies in a wide range of biological matrices.
Related equipment
Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Liver disease: 'Poo transplant' treatment trial launched
The Proteomics Facility is analysing samples for a new clinical trial, examining whether a "poo transplant" can benefit people suffering from advanced liver disease.
The Facility is actively involved in many research projects with King's College scientists and external groups, assisting with protein separation and mass spectrometry experiments to provide qualitative and quantitative information. Our growing list of peer-reviewed publications highlights the quality of the service we provide on a daily basis.
We encourage discussions for grant proposals and collaborations if you are considering involving proteomic research in your future work.
2023
Betancor, G., Bangham, M., Jeon, J.K., Shahm, K., Lynham, S., Jimenez-Guardeño, J.M., Malimm, M.H., (2022), MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation, mbio.2022.10.1128
Roetschke, H.P., Rodriguez-Hernandez, G., Cormican, J.A., Yang, X., Lynham, S., Mishto, M., Liepe, J., (2023), InvitroSPI and a large database of proteasome-generated spliced and non-spliced peptides, Sci Data.2023.10.1038.
Dobson, L., B. Barrell, W., Seraj, Z., Lynham, S., Yuan Wu, S., Krause, M., J. Liu, K., (2022), GSK3 and Lamellipodin balance lamellipodial protrusions and focal adhesion maturation in mouse neural crest migration, Cell Reports.2022.10.1101.
Faleeva, M., Ahmad, S., Lynham, S., Whitehead, M.C, Cox, S., Shanahan, C., (2023), Sox9 Regulates Extracellular Matrix Stiffness during Vascular Smooth Muscle Cell ageing by increased secretion of LH3 via extracellular vesicles, BioRxiv.2023.10.1101.
2022
Mishto M, Horokhovskyi Y, Cormican JA, Yang X, Lynham S, Urlaub H, Liepe J. (2022) Database search engines and target database features impinge upon the identification of post-translationally cis-spliced peptides in HLA class I immunopeptidomes. Proteomics 22(10).
Corsini PM, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle CJC, Mastroianni G, Dorgan B, Sewell LM, Lynham S, Iuga D, Franks WT, Jarvis J, Carpenter GH, Curtis MA, Bernadó P, Darbari VC, Garnett JA. (2022) Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation. NPJ Biofilms Microbiomes 8(1).
Qiongju Qiu, Giancarlo Abis, Florence Mattingly-Peck, Steven Lynham, Franca Fraternali, Maria R Conte. (2022) Allosteric Regulation of the Soluble Epoxide Hydrolase by Nitro Fatty Acids: a Combined Experimental and Computational Approach. J Mol Biol 434(17).
Betancor, G; Banhgham, M; Jeon, JK; Shah, K; Lynham, S; Jimenez-Guardeno, JM; Malim, M. (2022) MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation. mBio 13(4).
2021
Metier C, Dow J, Wootton H, Lynham S, Wren B, Wagner GK. (2021) Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes. Org Biomol Chem. 19(2).
Lisa F. Dawson, Johann Peltier, Catherine L. Hall, Mark A. Harrison, Maria Derakhshan, Helen A. Shaw, Neil F. Fairweather, and Brendan W. Wren. (2021) Extracellular DNA, cell surface proteins and c-di-GMP promote biofilm formation in Clostridioides difficile. Sci Rep. 11: 3244.
Vester SK, Beavil RL, Lynham S, Beavil AJ, Cunninghame Graham DS, McDonnell JM, Vyse TJ. (2021) Nucleolin acts as the receptor for C1QTNF4 and supports C1QTNF4-mediated innate immunity modulation. J. Biol Chem. 296:100513.
Lynham S, Freile FG, Puri NM, O'Reilly N, Mitchell GH, Wells TNC, Willcox M, Beatson R. (2021) Identification of chlorophyll a-b binding protein AB96 as a novel TGFβ1 neutralizing agent. Sci Rep. 11(1).
Watson L, Soliman TN, Davis K, Kelly J, Lockwood N, Yang X, Lynham S, Scott JD, Crossland V, McDonald NQ, Mann DJ, Armstrong A, Eggert U, Parker PJ. (2021) Co-ordinated control of the Aurora B abscission checkpoint by PKCε complex assembly, midbody recruitment and retention. Biochem J. 478(12).
Gilberto Betancor, Jose M. Jimenez-Guardeño, Steven Lynham, Robin Antrobus, Hataf Khan, Andrew Sobala, Matthew D. J. Dicks and Michael H. Malim. MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation. Nat. Microbiol. Jul 19.
Fauzy Nasher, Aidan J Taylor, Abdi Elmi, Burhan Lehri, Umer Z Ijaz, Dave Baker, Richard Goram, Steven Lynham, Dipali Singh, Richard Stabler, David J Kelly, Ozan Gundogdu, Brendan W Wren. (2021) MdaB and NfrA, two novel reductases important in the survival and persistence of the major enteropathogen Campylobacter jejuni. J Bacteriol. JB0042121.
Samata S Pandey, Adam Hartley, Mikhail Caga-Anan, Tareq Ammari, Ameer Hamid Ahmed Khan, Bao Anh Vu Nguyen, Chiari Kojima, Jon Anderson, Steven Lynham, Michael Johns, Dorian O Haskard, Ramzi Y Khamis. (2021) A Novel Immunoassay for Malondialdehyde-Conjugated Low-Density Lipoprotein Measures Dynamic Changes in the Blood of Patients Undergoing Coronary Artery Bypass Graft Surgery. Antioxidants (Basel) 10(8).
2020
Houghton JW, Carpenter G, Hans J, Pesaro M, Lynham S, Proctor G. (2020) Agonists of Orally Expressed TRP Channels Stimulate Salivary Secretion and Modify the Salivary Proteome. Mol Cell Proteomics. 19(10).
Metier CC, Peng J, Xu Y, Wootton H, Riesi V, Lynham S, Zadi S, Turner C, Wand ME, Mark Sutton J, Wagner GK. (2020) Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe. Bioorg Med Chem. 30:115900.
2019
Ashton NJ, Nevado-Holgado AJ, Barber IS, Lynham S, Gupta V, Chatterjee P, Goozee K, Hone E, Pedrini S, Blennow K, Schöll M, Zetterberg H, Ellis KA, Bush AI, Rowe CC, Villemagne VL, Ames D, Masters CL, Aarsland D, Powell J, Lovestone S, Martins R, Hye A. (2019) A plasma protein classifier for predicting amyloid burden for preclinical Alzheimer's disease. Sci Adv. 5(2).
Abis G, Charles RL, Kopec J, Yue WW, Atkinson RA, Bui TTT, Lynham S, Popova S, Sun YB, Fraternali F, Eaton P, Conte MR. (2019) 15-deoxy-Δ12,14-Prostaglandin J2 inhibits human soluble epoxide hydrolase by a dual orthosteric and allosteric mechanism. Commun Biol. 2:188.
Prysyazhna O, Wolhuter K, Switzer C, Santos C, Yang X, Lynham S, Shah AM, Eaton P, Burgoyne JR. (2019) Blood pressure-lowering by the antioxidant resveratrol is counterintuitively mediated by oxidation of cGMP-dependent protein kinase. Circulation. 140(2).
Doonan LB, Lynham S, Quinlan C, Ibiji SC, Winter CE, Padilla G, Jaimes-Becerra A, Morandini AC, Marques AC, Long PF. (2021) Venom Composition Does Not Vary Greatly Between Different Nematocyst Types Isolated from the Primary Tentacles of Olindias sambaquiensis (Cnidaria: Hydrozoa). Biol Bull. 237(1).
Mass spectrometry is a very sensitive technique that can detect analytes at very low concentrations. Contamination of preparations with keratin and plastisizer can be a problem as they can mask your protein of interest with their greater abundance.
To get the best possible LC-MS/MS analysis, we employ the following protocols. For further information and advice on sample preparation, please contact us and we will be happy to help.
In-gel digestion
Protocol for Proteins Separated by PAGE for MS analysis
Complex sample digestion
In-solution Digestion of Complex Biological Matrices
Protein identification
Determination of protein content from simple or complex mixtures and immunoprecipitations.
Post-translational modifications (PTM)
Analysis of modifications such as phosphorylation and ubiquitination with site localisation.
Protein quantitation
Relative quantification of proteomes using label-free or stable isotope labelling (TMT).
Biomarker discovery
Global protein discovery from complex mixtures and targeted analysis.
Facility staff
Proteomics Facility Manager
Proteomics Deputy Facility Manager
Mass Spectrometry Technician, Proteomics
The Proteomics Facility is equipped with state-of-the-art mass spectrometry technology combined with experienced scientists that can deliver the highest quality proteomic results.
We can provide a range of services from the simplest protein identification to very complex large scale biomarker discovery and verification studies in a wide range of biological matrices.
Related equipment
Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Liver disease: 'Poo transplant' treatment trial launched
The Proteomics Facility is analysing samples for a new clinical trial, examining whether a "poo transplant" can benefit people suffering from advanced liver disease.
The Facility is actively involved in many research projects with King's College scientists and external groups, assisting with protein separation and mass spectrometry experiments to provide qualitative and quantitative information. Our growing list of peer-reviewed publications highlights the quality of the service we provide on a daily basis.
We encourage discussions for grant proposals and collaborations if you are considering involving proteomic research in your future work.
2023
Betancor, G., Bangham, M., Jeon, J.K., Shahm, K., Lynham, S., Jimenez-Guardeño, J.M., Malimm, M.H., (2022), MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation, mbio.2022.10.1128
Roetschke, H.P., Rodriguez-Hernandez, G., Cormican, J.A., Yang, X., Lynham, S., Mishto, M., Liepe, J., (2023), InvitroSPI and a large database of proteasome-generated spliced and non-spliced peptides, Sci Data.2023.10.1038.
Dobson, L., B. Barrell, W., Seraj, Z., Lynham, S., Yuan Wu, S., Krause, M., J. Liu, K., (2022), GSK3 and Lamellipodin balance lamellipodial protrusions and focal adhesion maturation in mouse neural crest migration, Cell Reports.2022.10.1101.
Faleeva, M., Ahmad, S., Lynham, S., Whitehead, M.C, Cox, S., Shanahan, C., (2023), Sox9 Regulates Extracellular Matrix Stiffness during Vascular Smooth Muscle Cell ageing by increased secretion of LH3 via extracellular vesicles, BioRxiv.2023.10.1101.
2022
Mishto M, Horokhovskyi Y, Cormican JA, Yang X, Lynham S, Urlaub H, Liepe J. (2022) Database search engines and target database features impinge upon the identification of post-translationally cis-spliced peptides in HLA class I immunopeptidomes. Proteomics 22(10).
Corsini PM, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle CJC, Mastroianni G, Dorgan B, Sewell LM, Lynham S, Iuga D, Franks WT, Jarvis J, Carpenter GH, Curtis MA, Bernadó P, Darbari VC, Garnett JA. (2022) Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation. NPJ Biofilms Microbiomes 8(1).
Qiongju Qiu, Giancarlo Abis, Florence Mattingly-Peck, Steven Lynham, Franca Fraternali, Maria R Conte. (2022) Allosteric Regulation of the Soluble Epoxide Hydrolase by Nitro Fatty Acids: a Combined Experimental and Computational Approach. J Mol Biol 434(17).
Betancor, G; Banhgham, M; Jeon, JK; Shah, K; Lynham, S; Jimenez-Guardeno, JM; Malim, M. (2022) MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation. mBio 13(4).
2021
Metier C, Dow J, Wootton H, Lynham S, Wren B, Wagner GK. (2021) Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes. Org Biomol Chem. 19(2).
Lisa F. Dawson, Johann Peltier, Catherine L. Hall, Mark A. Harrison, Maria Derakhshan, Helen A. Shaw, Neil F. Fairweather, and Brendan W. Wren. (2021) Extracellular DNA, cell surface proteins and c-di-GMP promote biofilm formation in Clostridioides difficile. Sci Rep. 11: 3244.
Vester SK, Beavil RL, Lynham S, Beavil AJ, Cunninghame Graham DS, McDonnell JM, Vyse TJ. (2021) Nucleolin acts as the receptor for C1QTNF4 and supports C1QTNF4-mediated innate immunity modulation. J. Biol Chem. 296:100513.
Lynham S, Freile FG, Puri NM, O'Reilly N, Mitchell GH, Wells TNC, Willcox M, Beatson R. (2021) Identification of chlorophyll a-b binding protein AB96 as a novel TGFβ1 neutralizing agent. Sci Rep. 11(1).
Watson L, Soliman TN, Davis K, Kelly J, Lockwood N, Yang X, Lynham S, Scott JD, Crossland V, McDonald NQ, Mann DJ, Armstrong A, Eggert U, Parker PJ. (2021) Co-ordinated control of the Aurora B abscission checkpoint by PKCε complex assembly, midbody recruitment and retention. Biochem J. 478(12).
Gilberto Betancor, Jose M. Jimenez-Guardeño, Steven Lynham, Robin Antrobus, Hataf Khan, Andrew Sobala, Matthew D. J. Dicks and Michael H. Malim. MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation. Nat. Microbiol. Jul 19.
Fauzy Nasher, Aidan J Taylor, Abdi Elmi, Burhan Lehri, Umer Z Ijaz, Dave Baker, Richard Goram, Steven Lynham, Dipali Singh, Richard Stabler, David J Kelly, Ozan Gundogdu, Brendan W Wren. (2021) MdaB and NfrA, two novel reductases important in the survival and persistence of the major enteropathogen Campylobacter jejuni. J Bacteriol. JB0042121.
Samata S Pandey, Adam Hartley, Mikhail Caga-Anan, Tareq Ammari, Ameer Hamid Ahmed Khan, Bao Anh Vu Nguyen, Chiari Kojima, Jon Anderson, Steven Lynham, Michael Johns, Dorian O Haskard, Ramzi Y Khamis. (2021) A Novel Immunoassay for Malondialdehyde-Conjugated Low-Density Lipoprotein Measures Dynamic Changes in the Blood of Patients Undergoing Coronary Artery Bypass Graft Surgery. Antioxidants (Basel) 10(8).
2020
Houghton JW, Carpenter G, Hans J, Pesaro M, Lynham S, Proctor G. (2020) Agonists of Orally Expressed TRP Channels Stimulate Salivary Secretion and Modify the Salivary Proteome. Mol Cell Proteomics. 19(10).
Metier CC, Peng J, Xu Y, Wootton H, Riesi V, Lynham S, Zadi S, Turner C, Wand ME, Mark Sutton J, Wagner GK. (2020) Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe. Bioorg Med Chem. 30:115900.
2019
Ashton NJ, Nevado-Holgado AJ, Barber IS, Lynham S, Gupta V, Chatterjee P, Goozee K, Hone E, Pedrini S, Blennow K, Schöll M, Zetterberg H, Ellis KA, Bush AI, Rowe CC, Villemagne VL, Ames D, Masters CL, Aarsland D, Powell J, Lovestone S, Martins R, Hye A. (2019) A plasma protein classifier for predicting amyloid burden for preclinical Alzheimer's disease. Sci Adv. 5(2).
Abis G, Charles RL, Kopec J, Yue WW, Atkinson RA, Bui TTT, Lynham S, Popova S, Sun YB, Fraternali F, Eaton P, Conte MR. (2019) 15-deoxy-Δ12,14-Prostaglandin J2 inhibits human soluble epoxide hydrolase by a dual orthosteric and allosteric mechanism. Commun Biol. 2:188.
Prysyazhna O, Wolhuter K, Switzer C, Santos C, Yang X, Lynham S, Shah AM, Eaton P, Burgoyne JR. (2019) Blood pressure-lowering by the antioxidant resveratrol is counterintuitively mediated by oxidation of cGMP-dependent protein kinase. Circulation. 140(2).
Doonan LB, Lynham S, Quinlan C, Ibiji SC, Winter CE, Padilla G, Jaimes-Becerra A, Morandini AC, Marques AC, Long PF. (2021) Venom Composition Does Not Vary Greatly Between Different Nematocyst Types Isolated from the Primary Tentacles of Olindias sambaquiensis (Cnidaria: Hydrozoa). Biol Bull. 237(1).
Mass spectrometry is a very sensitive technique that can detect analytes at very low concentrations. Contamination of preparations with keratin and plastisizer can be a problem as they can mask your protein of interest with their greater abundance.
To get the best possible LC-MS/MS analysis, we employ the following protocols. For further information and advice on sample preparation, please contact us and we will be happy to help.
In-gel digestion
Protocol for Proteins Separated by PAGE for MS analysis
Complex sample digestion
In-solution Digestion of Complex Biological Matrices
Contact us
2nd Floor, James Black Centre Denmark Hill Campus.