Electron tomography is a technique that involves tilting a specimen within an electron microscope to capture images from multiple angles, allowing for the reconstruction of a three-dimensional image

This information is then aligned and assembled as a 3D image stack. A weighted back-projection is then used to generate a 3D tomographic volume revealing 3D organisation of organelle and their interactions.

Equipment available

• JEOL JEM F200
• JEOL JEM 1400Flash