We employ advanced single molecule imaging approaches to understand enveloped virus fusion mechanics. We use a highly interdisciplinary approach that combines, virology, molecular biology, advanced biophysics live cells imaging and single molecule spectroscopy to define virus fusion molecular machines. In particular we investigate a number of enveloped viruses during the first steps of infection (virus entry and fusion) that comprise Human Immunodeficiency Virus 1 (HIV-1), Ebola (EBOV) and SARS Corona viruses (SARS CoV 2).