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The Genome Stability group is based at King’s College London School of Cancer & Pharmaceutical Sciences. Our group uses genetic screens and a range of molecular and cell biology techniques to elucidate the molecular mechanisms of DNA damage repair. With a specific focus on understanding how the DDR can be targeted alongside radiotherapy to improve therapy response and overcome resistance.
We are seeking an enthusiastic, self-motivated PhD student to join our team. You will work closely with other lab members and have the opportunity to acquire skills in a wide range of techniques such as mammalian cell culture, live-cell immaging and whole genome CRISPR screens.
Tumour heterogeneity, characterized by genetic instability and diverse cell populations, poses a significant challenge to effective cancer treatment. The varying sensitivity of these populations to targeted therapies often leads to the persistence of therapy-resistant cells, hampering long-term treatment success. Understanding the intricate interactions between different cell populations within tumours is essential for overcoming this obstacle.
This PhD project will investigate the phenomenon of bystander apoptosis—where neighbouring cells undergo apoptosis in response to signals from dying cells. In cancer, this effect can extend the impact of therapeutic agents beyond targeted cells, potentially enhancing treatment efficacy. Conversely, controlling this process may help protect surrounding non-tumour tissue, reducing collateral damage during treatment.
The student will evaluate the relative the contribution of bystander apoptosis in difference cancer and non-cancer cellular subtypes and use the CRISPR screening platform established in the Hewitt lab to uncover the molecular mechanisms that govern this cell-to-cell communication.
This project will provide the student training in a diverse set of skills in molecular and cell biology, including CRISPR screening and gene editing, cell line generation and live-cell imaging
Interested applicants should contact Dr Graeme Hewitt to arrange applying now (graeme.hewitt@kcl.ac.uk)
Start date: 1 February 2025 (can be discussed with Dr Hewitt).
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